Fig. 7.

Side-by-side comparison of a virtual slice from the microCT. (A) Virtual slice from the microCT and back scattered SEM image (B) collected from the block surface revealed by the 3View. Gross morphology such as small capillaries (blue, circled) aid in registering the two datasets. Small differences in registration between the images due to slight miss-registration (circled red). (C) Virtual section from the microCT of the worm head visualised in IMOD using ‘Slicer’. (D) SBF-SEM image of the same region collected live in Digital Micrograph. Videos of the corresponding stacks of the virtual (CT) and real slices (SEM) side by side are included in the supplementary material. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)