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. 2019 Mar 25;90:1671. doi: 10.4102/jsava.v90i0.1671

TABLE 2.

Details of sequences and amplicon size of primers used in this study for polymerase chain reaction and sequencing spanning the viral capsid protein 2 gene region.

Primer set Name Sequence (5’–3’) Amplicon size (base pair)
1 CPV_EF GCCGGTGCAGGACAAGTA 421
M2R AGTTGCCAATCTCCTGGATT
2 PV_VP2-1§ GAAAACGGATGGGTGGAAATC 405
PV_VP2-2§ AGCTGCTGGAGTAAATGGCATAGT
3 PV_VP2-3§ TAAAGACTGTTTCAGAATCTGCTACTCA 424
PV_VP2-4§ AGAAATGGTGGTAAGCCCAATG
4 PV_VP2-5§ ATACTGGAACTAGTGGCACACC 437
PV_VP2-5ii ATTTAAAACACCTATTGCAGCAGGAC
5 PV_VP2-5iiR GTCCTGCTGCAATAGGTGTTTTAAAT 440
PV_VP2-8§ GCATCAGGATCATATTCATTTGTTAA
6 Primer 22 F†† TGTCAAAATAATTGTCCTGG 564
CPV_JS2R CAACCCACACCATAACAACA

PV, Parvovirus; VP2, viral capsid protein 2.

, Meers et al. 2007.

, Steinel et al. 2000.

§

, Wasieri et al. 2009.

, Helene Brettschneider.

††

, Horiuchi et al. 1996.