Fig. 5.

CD4 depletion impairs tumor growth and CD8⁺ T-cell activity. a Tumor volume was measured over 14 days. b T cells from tumors were restimulated ex vivo and the expression of IFNγ and granzyme B was assayed by flow cytometry on day 14. Plots shown are gated on live, CD3⁺, and CD8⁺ lymphocytes. c The number of CD8⁺ T cells was normalized to tumor mass. d The number of CD8⁺IFNγ⁺GZB⁺ cells was normalized to tumor mass. This experiment had five mice per group and was performed three times with similar results. Error bars represent standard error of the mean. Statistical difference is expressed as *p < 0.05, **p < 0.005, and ^#p < 0.0005