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. 2019 Apr 4;216(5):1135–1153. doi: 10.1084/jem.20181652

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© 2019 Greaves et al.

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Figure 7. — Activation and plasmablast differentiation of P110* autoreactive B cells in response to self-antigen. (A–C) Frequencies and representative analyses of B220⁺ (or B220⁺GFP⁺) B cells expressing CD69 (A), CD86 (B), or CD138 (C) in the spleen of autoreactive (AUT) mb1Cre (open circles and black dashed lines, n = 11–14) or P110*-mb1Cre (close red squares and red intact lines, n = 9–10) mice and nonautoreactive (NA) mb1Cre (closed black circles and black intact lines, n = 7) or P110*-mb1Cre (close blue squares and blue intact lines, n = 6) mice. Data are from three or more experiments for each group of mice. (D) Top: Change in the expression of CD69 and CD86 (MFI fold change) on B220⁺CD23⁺ spleen B cells relative to total B220⁺ cells in individual autoreactive and nonautoreactive mb1Cre and P110*-mb1Cre mice. Bottom: Representative analyses of CD69 on B220⁺ and B220⁺CD23⁺ cells in P110* autoreactive (left) and nonautoreactive (right) mice. Number of mice per group and experiments are as listed for A–C. Data in all panels show the mean ± SEM. P values were calculated using a one-tailed Mann–Whitney U test. *, P ≤ 0.05; ***, P ≤ 0.001; ****, P ≤ 0.0001; ns, not significant.