Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Mouse anti-ChaT monoclonal | DSHB | ChAT4B1 | 1:50 diltuion |
| Antibody | Rabbit anti-GABA polyclonal | Sigma | A2052 | 1:1000 dilution |
| Antibody | Mouse anti-GABA monoclonal | Sigma | A0310 | 1:1000 dilution |
| Antibody | Rabbit antiVGlut polyclonal | Daniels et al., 2004 | 1:10000 dilution | |
| Antibody | Mouse anti-BRP monoclonal | DSHB | nc82 | 1:50 diltuion |
| Antibody | Chicken anti-GFP polyclonal | Life Tech. | A10262 | 1:1000 dilution |
| Antibody | Rabbit anti-DsRED polyclonal | TAKARA | 632496 | 1:500 dilution |
| Antibody | Rabbit anti-Vg polyclonal | Gift from Sean Carroll | 1:500 dilution | |
| Antibody | Guinea pig anti-Ems polyclonal | Gift from U. Walldorf | 1:300 dilution | |
| Antibody | Rabbit anti-Msh polyclonal | Gift from Chris Doe | 1:500 dilution | |
| Antibody | Rabbit anti-Unc-4 polyclonal | Gift from James Skeath | 1:1000 dilution | |
| Antibody | Guinea pig anti-Dbx polyclonal | Gift from James Skeath | 1:1000 dilution | |
| Antibody | Rabbit anti-HB9 polyclonal | Gift from James Skeath | 1:1000 dilution | |
| Antibody | Guinea pig anti-HB9 polyclonal | Gift from James Skeath | 1:1000 dilution | |
| Antibody | Rat anti-Nkx6 polyclonal | Gift from James Skeath | 1:1000 dilution | |
| Antibody | Guinea pig anti-Lim3 polyclonal | Gift from James Skeath | 1:1000 dilution | |
| Antibody | Rat anti-Islet polyclonal | Gift from James Skeath | 1:1000 dilution | |
| Antibody | Rabbit anti-Dichaete polyclonal | Gift from James Skeath | 1:1000 dilution | |
| Antibody | Rabbit anti-Nmr1 polyclonal | Gift from James Skeath | 1:1000 dilution | |
| Antibody | Mouse anti-En monoclonal | DSHB | 4D9 | 1:5 dilution |
| Antibody | Mouse anti-Acj6 monoclonal | DSHB | Acj6 | 1:100 dilution |
| Antibody | Mouse anti-Eve monoclonal | DSHB | 3C10 | 1:25 dilution |
| Antibody | goat anti-rabbit Alexa Fluor 488 | Life Technologies | A-11034 | 1:500 dilution |
| Antibody | goat anti-rabbit Alexa Fluor 568 | Life Technologies | A-11011 | 1:500 dilution |
| Antibody | goat anti-rabbit Alexa Fluor 633 | Life Technologies | A-21070 | 1:500 dilution |
| Antibody | goat anti-rabbit Alexa Fluor 405 | Life Technologies | A-31556 | 1:500 dilution |
| Antibody | goat anti-chicken Alexa Fluor 488 | Life Technologies | A-11039 | 1:500 dilution |
| Antibody | goat anti-mouse Alexa Fluor 488 | Life Technologies | A-11001 | 1:500 dilution |
| Antibody | goat anti-mouse Alexa Fluor 568 | Life Technologies | A-11004 | 1:500 dilution |
| Antibody | goat anti-mouse Alexa Fluor 633 | Life Technologies | A-21050 | 1:500 dilution |
| Antibody | goat anti-mouse Alexa Fluor 405 | Life Technologies | A-31553 | 1:500 dilution |
| Antibody | goat anti-guinea pig Alexa Fluor 488 | Life Technologies | A-11073 | 1:500 dilution |
| Antibody | goat anti-guinea pig Alexa Fluor 568 | Life Technologies | A-11075 | 1:500 dilution |
| Antibody | goat anti-guinea pig Alexa Fluor 633 | Life Technologies | A-21105 | 1:500 dilution |
| Antibody | goat anti-rat Alexa Fluor 488 | Life Technologies | A-11006 | 1:500 dilution |
| Antibody | goat anti-rat Alexa Fluor568 | Life Technologies | A-11077 | 1:500 dilution |
| Genetic reagent (D. melanogaster) | VGlut-GAL4 | Bloomington Drosophila Stock Center | RRID:BDSC_60312 | |
| Genetic reagent (D. melanogaster) |
VGlutDBD | Bloomington Drosophila Stock Center |
RRID:BDSC_60313 | |
| Genetic reagent (D. melanogaster) | VGlut-LexA | Bloomington Drosophila Stock Center |
RRID:BDSC_60314 | |
| Genetic reagent (D. melanogaster) | ChAT-GAL4 | Bloomington Drosophila Stock Center | RRID:BDSC_60317 | |
| Genetic reagent (D. melanogaster) | ChAT-DBD | Bloomington Drosophila Stock Center |
RRID:BDSC_60318 | |
| Genetic reagent (D. melanogaster) |
ChAT-LexA | Bloomington Drosophila Stock Center | RRID:BDSC_60319 | |
| Genetic reagent (D. melanogaster) | gad1-AD | Bloomington Drosophila Stock Center | RRID:BDSC_60322 | |
| Genetic reagent (D. melanogaster) | gad1-LexA | Bloomington Drosophila Stock Center | RRID:BDSC_60324 | |
| Genetic reagent (D. melanogaster) |
Tub-AD | Bloomington Drosophila Stock Center | RRID:BDSC_60295 | |
| Genetic reagent (D. melanogaster) |
Tub-DBD | Bloomington Drosophila Stock Center | RRID:BDSC_60298 | |
| Genetic reagent (D. melanogaster) |
SS20873 | This study | ||
| Chemical compound, drug |
Paraformaldehyde | EMS | 15713 | |
| Chemical compound, drug | Vectashield | Vector | H-1000 | |
| Chemical compound, drug |
Prolong Diamond | Molecular Probes | P36961 | |
| Recombinant DNA reagent |
pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70 | addgene | RRID:Addgene_62904 | |
| Recombinant DNA reagent | pBS-KS-attB2-SA(1)-T2A- Gal4DBD-Hsp70 |
addgene | RRID:Addgene_62903 | |
| Recombinant DNA reagent |
pBS-KS-attB2-SA(0)-T2A-Gal4DBD-Hsp70 | addgene | RRID:Addgene_62902 | |
| Recombinant DNA reagent |
pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70 | addgene | RRID:Addgene_62915 | |
| Recombinant DNA reagent | pBS-KS-attB2- SA(1)-T2A- p65AD-Hsp70 |
addgene | RRID:Addgene_62914 | |
| Recombinant DNA reagent |
pBS-KS-attB2-SA(0)-T2A-p65AD-Hsp70 | addgene | RRID:Addgene_62912 |