Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) |
Fasnlox/lox | PMID:16054078 | MGI:3765070 | Dr. Clay F. Semenkovich, Washington University, St. Louis USA |
| Genetic reagent (M. musculus) | PdgfraCreERT2 | PMID:18849983 | MGI:3832569 | Dr. William D. Richardson, University College London, London UK |
| Genetic reagent (M. musculus) | Olig2Cre | Jackson Laboratory | Stock#:011103; MGI:3810299 | PMID:18691547 |
| Genetic reagent (M. musculus) | Rosa26-loxPstoploxP-YFP | Jackson Laboratory | Stock#:006148; MGI:3621481 | PMID:11299042 |
| Sequence-based reagent | Genotyping primer: Fasn forward |
PMID:29434029 | 5'-GGATAGCTGTGTAGTGTAACCAT-3’ | |
| Sequence-based reagent | Genotyping primer: Fasn reverse | PMID:29434029 | 5’-GGTCACCCAGCAGGAAAGGGC- 3’ | |
| Sequence-based reagent | Genotyping primer: Cre forward | PMID: 29434029 | 5’-TTCCCGCAGAACCTGAAGATGTTCG-3’ | |
| Sequence-based reagent | Genotyping primer: Cre reverse | PMID: 29434029 | 5’-GGGTGTTATAAGCAATCCCCAGAAATG-3’ | |
| Sequence-based reagent | Genotyping primer: Rosa26-loxPstoploxP-YFP forward | PMID: 28522536 | 5’-AAAGTCGCTCTGAGTTGTTAT-3’ | |
| Sequence-based reagent | Genotyping primer: Rosa26-loxPstoploxP-YFP reverse transgenic | PMID: 28522536 | 5’-GCGAAGAGTTTGTCCTCAACC-3’ | |
| Sequence-based reagent | Genotyping primer: Rosa26-loxPstoploxP-YFP reverse wildtype | PMID: 28522536 | 5’-GGAGCGGGAGAAATGGATATG-3’ | |
| Sequence- based reagent |
qRT-PCR primer: SOX10 Forward | This paper | 5’-CCGACCAGTACCCTCACCT-3’ | |
| Sequence-based reagent | qRT-PCR primer: SOX10 Reverse | This paper | 5’- TCAATGAAGGGGCGCTTGT-3’ | |
| Sequence-based reagent | qRT-PCR primer: MYRF Forward | This paper | 5’-ATGGAGGTGGTGGACGAGAC-3’ | |
| Sequence-based reagent | qRT-PCR primer:MYRF Reverse | This paper | 5’-GGCGTCCTCTTTGCCAATGT-3’ | |
| Sequence- based reagent |
qRT-PCR primer: MBP Forward | This paper | 5’-ACACGAGAACTACCCATTATGGC-3’ | |
| Sequence- based reagent |
qRT-PCR primer: MBP Reverse |
This paper | 5’-CCAGCTAAATCTGCTGAGGGGA-3’ | |
| Sequence- based reagent |
qRT-PCR primer: Actin Forward |
PMID: 28880149 | 5’-GTCCACACCCGCCACC-3’ | |
| Sequence- based reagent |
qRT-PCR primer: Actin Reverse | PMID: 28880149 | 5’-GGCCTCGTCACCCACATAG-3’ | |
| Antibody | Rabbit polyclonal anti-cleaved Caspase 3 | Cell signaling Technology, Danvers, MA, USA |
Cat# 9661; RRID:AB_2341188 | 1:500 dilution |
| Antibody | Mouse monoclonal anti-CC1 | Merck Millipore, Billerica, MA, USA | Cat; # OP80; RRID:AB_2057371 | Development: 1:200 dilution; Remyelination 1:300 dilution |
| Antibody | rabbit polyclonal anti-FASN | Abcam, UK | Cat# Ab22759; RRID:AB_732316 |
1:200 dilution |
| Antibody | Chicken polyclonal anti-GFP | Abcam, UK | Cat# Ab13970; RRID:AB_300798 | 1:1000 dilution |
| Antibody | mouse monoclonal anti-ki67 | Dako Agilent, Santa Clara, CA, USA |
Cat# m7249; clone MIB-5; RRID:AB_2250503 | 1:200 dilution |
| Antibody | rat monoclonal anti-MBP | Serotec/BioRad Laboratories, Hercules, CA, USA |
Cat# MCA409S; RRID:AB_325004 | Development: 1:200 dilution; Remyelination 1:300 dilution |
| Antibody | Goat polyclonal anti-Olig2 | R and D Systems, Minneapolis, MN, USA |
Cat# AF2418; RRID:AB_2157554 | 1:25 dilution |
| Antibody | mouse monoclonal anti-Olig2 | Merck Millipore, Billerica, MA, USA | Cat# MABN50; clone 211F1.1; RRID:AB_10807410 | 1:1000 dilution |
| Antibody | rabbit polyclonal anti-Olig2 | Merck Millipore, Billerica, MA, USA | Cat# AB9610; RRID:AB_570666 | Development: 1:500 dilution; Remyelination 1:400 dilution |
| Antibody | Rabbit monoclonal anti-PDGFRα | Cell signaling Technology, Danvers, MA, USA | Cat# 3174; RRID:AB_2162345 | 1:500 dilution |
| Commercial assay or kit | Click-iT EdU Assay |
Thermo Fisher Scientific, Waltham, MA, USA |
Cat# C10337 | |
| Commercial assay or kit | Mouse blocking reagent | Vector Laboratories, Burlingame, CA, USA | Cat# MKB-2213 | |
| Commercial assay or kit | Streptavidin/Biotin blocking Kit | Vector Laboratories, Burlingame, CA, USA | Cat# SP-2002 | |
| Commercial assay or kit | Qiagen MiniKit (RNeasy Mini Kit) | Qiagen, Hilden, Germany | Cat# 74104 | |
| Commercial assay or kit | TruSeq Stranded mRNA Sample Prep Kit | Illumina, San Diego, CA, USA | Cat# 20020594 | |
| Commercial assay or kit |
Maxima RT-Kit | Thermo Fisher Scientific, Waltham, MA, USA | Cat# K1641 | |
| Chemical compound, drug | Lysolecithin | Sigma-Aldrich, Sant Louis, MO, USA | Cat# L4129 | |
| Chemical compound, drug | Tamoxifen | Sigma-Aldrich, Sant Louis, MO, USA | Cat# T5648 | |
| Chemical compound, drug | Lipid standards | Avanti Polar Lipids, Alabaster, AL, USA | ||
| Software, algorithm | Lipid Data Analyzer software | PMID: 29058722 | ||
| Software, algorithm | Photoshop CS5 or CS6 | Adobe | ||
| Software, algorithm |
FIJI | ImageJ (http://imagej.nih.gov/ij/) | ||
| Software, algorithm | STAR Aligner(v2.5.1b) | PMID: 23104886 | ||
| Software, algorithm | RSEM (v1.2.22) | PMID: 21816040 | ||
| Software, algorithm | EdgeR | PMID: 19910308 | ||
| Software, algorithm | Metacore (vs6.33) | Thomson Reuters | ||
| Other | Standard diet (STD) | KLIBA NAFAG, Provimi KLIBA, Switzerland | Cat# 3437 | |
| Other | High fat diet (HFD) | KLIBA NAFAG, Provimi KLIBA, Switzerland | Cat# 2127 |