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(A) Sequence of the primer (FAM-labeled, 12-mer) and the template (19-mer); (B) SDS-PAGE (10%, Bis-Tris) of AGT-oligonucleotide crosslink reaction at the N2-position of dG. Lane 1: Precision Plus Protein dual color standard; Lane 2: AGT-oligonucleotide crosslink reaction after 1 h; Lane 3: AGT-oligonucleotide crosslink reaction after 3 h; Lane 4: AGT protein (21.9 KDa).
