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. 2019 Feb 26;151(5):660–669. doi: 10.1085/jgp.201812199

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© 2019 Solís and Russell

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Figure 2. — CapZ-GFP dynamics increase with deletion of the β-tentacle determined by the FRAP kinetic constant. (A) Fluorescence recovery over a period of 15 min after photobleaching GFP (green) a single Z-disc in the middle of three sarcomeres in an NRVM grown on glass infected with virus for CapZβ1-GFP (upper) or CapZβ1ΔC-GFP (lower). Scale bar, 1 µm. (B) Fluorescence recovery intensity traces of CapZβ1-GFP or CapZβ1ΔC-GFP are used to calculate the fluorescence recovery rates (k_FRAP). (C) Deletion of the β-tentacle increases CapZ-GFP dynamics (mean ± SEM, n = 4; *, P < 0.05).