Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 26;151(5):660–669. doi: 10.1085/jgp.201812199

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Solís and Russell

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — PIP2 localization in the Z-lines in neonatal myocytes grown on varying stiffness and with neomycin treatment. (A) NRVMs plated on PAA substrates (10 kPa and 100 kPa) or glass were fixed and stained for α-actinin (red) and PIP2 (green). Dashed squares are enlarged in lower panels to show separate α-actinin and PIP2 fluorescence images. Scale bars, 10 µm. (B) Line scans corresponding to white lines in A depict PIP2 (green) localization in the Z-lines represented by α-actinin (red). (C) Peak PIP2 to α-actinin intensity ratio quantifies the effect of neomycin and stiffness on PIP2 localization in the Z-lines (mean ± SEM, n = 15–21; *, P < 0.05).