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. 2019 Jan 14;151(5):670–679. doi: 10.1085/jgp.201812214

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© 2019 Gregorich et al.

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Figure 3. — The expression of MHC isoforms, but not other thin/thick filament regulatory proteins, is altered in ENH^−/− mouse myocardium. (A) Representative silver-stained gels showing expression profile of myosin heavy chain isoforms (top) and associated quantification (bottom) in ENH^+/+ and ENH^−/− trabeculae. (B) Zoomed-in deconvoluted mass spectrum showing ENH protein species detected in ENH^+/+, but not ENH^−/−, mouse myocardium. Star denotes potential acetylated form of _pENH3b (ΔM = 42.00 Da). (C–J) Top-down proteomics quantification of thin/thick filament protein expression in the ventricular myocardium of ENH^+/+ and ENH^−/− mice. Each data point is the mean and error bar the SEM.