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. Author manuscript; available in PMC: 2020 Jun 15.
Published in final edited form as: Anal Biochem. 2019 Mar 30;575:40–43. doi: 10.1016/j.ab.2019.03.020

Figure 1.

Figure 1.

Enzymatic Derivatization of Glucose and Glycerol. (A) Derivatization Schematic. Glucose and glycerol are converted to glucose-6-phosphate (Glc-6-P) and sn-glycerol-3-phosphate (Gro-3-P) using hexokinase and glycerokinase and ATP. (B) Extracted ion chromatograms comparing the detection of the underivatized glucose (Glc) (m/z 179.0556) to derivatized of Glc-6-P (m/z 259.0224). The Glc intensity is enhanced 5X. (C) Extracted ion chromatograms comparing the detection of the underivatized Glycerol (Glo) (m/z 105.0190) to derivatized of Gro-3-P (m/z 171.0063). (D) Standard curves of Glc and Glo at 0μM, 5μM, 10μM, 20μM, 30μM, 40μM, 50μM, 75μM, 100μM, 250μM, 500μM, 1000μM, and 2000μM concentrations. R2=0.9978 and 0.9987 for Glc-6-P and Gro-3-P respectively.