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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Exp Eye Res. 2019 Mar 5;182:1–9. doi: 10.1016/j.exer.2019.02.024

Figure 2: Subcellular localization of SIRT3 and SIRT5.

Figure 2:

Mouse lens epithelial cells were cultured, fractionated as described in Material and Methods and immunoblotted using histone H3 antibodies (A), SOD2 (B) and β-actin (C) antibodies. The isolated fractions were Western blotted using SIRT3 (D) and SIRT5 (E) antibodies. Arrows in panels D and E indicate SIRT3 and SIRT5, respectively. C, cytosolic; M, mitochondrial; N, nuclear. Mr, molecular weight markers.