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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: J Immunol. 2019 Apr 15;202(10):3020–3032. doi: 10.4049/jimmunol.1801456

Figure 1.

Figure 1.

Injury Induces Expansion of Immature HSPC Populations. C57BL/6 mice (n=11–12) were subjected to polytrauma. 24 hours after injury, bone marrow was isolated from injured mice and naïve controls and analyzed by flow cytometry. A) Representative FACS plots for identification of hematopoietic progenitor cells. Early progenitor cells were gated as live, lineage-negative, CD117+ (cKit) Sca-1+ (KSL) cells with the following phenotypes: CD150+ CD48− (LT-HSC), CD34+ CD135− (ST-HSC), CD34+ CD135+ (MPP). The total number of cells per femur (B, mean+/− SD) and the frequency of each population among live bone marrow cells are shown (C, individual values shown, horizontal bar/whisker represent mean+/−SD). Data are combined from 3 independent series of experiments. *= p<0.05 by Mann-Whitney U test.