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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: J Immunol. 2019 Apr 15;202(10):3020–3032. doi: 10.4049/jimmunol.1801456

Figure 4.

Figure 4.

Injury is Associated with Increased Myeloid Cell Numbers in the Bone Marrow and Spleen. WT mice were subjected to polytrauma; bone marrow (A), blood (B) and spleens (C) were harvested 10 or 21 days after injury (or from naïve animals) and analyzed by flow cytometry. Absolute numbers of cells (left y axis) and frequency among CD45+ cells (right y axis) are shown; horizontal bar/whisker represent mean+/−SD). Data are pooled from 2–3 independent experiments per time point. * = p<0.05 by Mann-Whitney U test. Cell subset gating was done as shown in Supplemental Figure 4; splenic dendritic cells were identified as CD3CD19NK1.1CD11b+CD11c+ cells.