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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: J Immunol. 2019 Apr 15;202(10):3020–3032. doi: 10.4049/jimmunol.1801456

Figure 6.

Figure 6.

MyD88 Drives G-CSF Production and Hematopoietic Progenitor Expansion after Injury. WT (C57BL/6) and MyD88−/− mice were subjected to polytrauma. 24 hours after injury bone marrow and plasma was harvested and G-CSF measured by cytometric bead array (CBA). A. Representative FACS plots showing progenitors in WT and MyD88−/− mice 24 hours after injury. B. Plasma G-CSF from WT and MyD88−/− mice 24 hours after injury C. HSPC frequency was assayed by flow cytometry; *= p<0.05 by Mann-Whitney U test. D. 104 bone marrow cells were cultured in methylcellulose media. The number of cell-colony-forming units was counted at day 7 of culture; (mean+/−SEM, n=3/group, *=p<0.05 by t-test). B,C: Data are pooled from 3 independent experiments. Individual values shown, horizontal bar/whisker represent mean+/−SD). C: Data are pooled from 2 independent experiments.