Skip to main content
. Author manuscript; available in PMC: 2020 May 6.
Published in final edited form as: Curr Biol. 2019 Apr 25;29(9):1460–1470.e4. doi: 10.1016/j.cub.2019.03.066

Figure 6. The ability of hyperactive ftsW to suppress loss of fzlA is conserved.

Figure 6.

(A) Micrographs of PG synthase hyperactive mutant cells ± FzlA in Agrobacterium tumefaciens. fzlA was induced where indicated with IPTG and depleted where indicated upon removal of IPTG, then grown for 16 hours on agarose pads. White arrowheads mark ectopic poles at midcell. Scale bar: 2 μm.

(B) Spot dilutions (diluted ten-fold) of the indicated strains grown in the presence or absence of IPTG to control fzlA expression, in A. tumefaciens.

(C) Phase contrast time-lapse microscopy images depicting WT and PG synthase hyperactive mutant cells depleted of FzlA over time. White arrowheads mark ectopic poles at midcell. Scale bar: 2 μm.

Strain key (A. tumefaciens): WT (PBA44)[55]; AfzlA PlacfzlA (PBA199); AfzlA PlacfzlA ftsWF137L (PBA232).