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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: J Immunol. 2019 Apr 8;202(10):2837–2842. doi: 10.4049/jimmunol.1801220

FIGURE 2.

FIGURE 2.

Tal1 limits lymphoid gene transcription in ST-HSC and LMPP. (A) Quantitative RT-PCR analysis of Rag1 and Dntt mRNA in sorted LMPPs from Ctrl and Tal1−/− mice. Data are mean ± s.e.m from 3 independent experiments combined. *P < 0.05. (B) FACS showing CD135 and Rag2-GFP expression in LSKs. The frequency of Rag2-GFP+ cells is indicated. (C) Percent of LSKs expressing Rag2-GFP (left) and the number of Rag2-GFP+ LSKs (right) in the BM of Ctrl (black) and Tal1−/− (blue) mice. Each circle represents one mouse, line connects mice from the same experiment. (D) FACS showing CD34 and CD135 on LSKs for identification of ST-HSC (gated area) and Rag2-GFP expression (right panels) in ST-HSC as compared to LT-HSCs in Ctrl and Tal1−/− mice. (E) Summary of the number of Rag2-GFP+ ST-HSC in Ctrl and Tal1−/− mice. Each circle represents one mouse, line connects mice from the same experiment. (F) FACS showing CD135 and CD117 on LSK with LMPP gated (left panel) and Rag2-GFP expression in LMPP compared to LT-HSCs (right panel) in Ctrl and Tal1−/− mice. (G) Number of Rag2-GFP+ LMPP in the BM of Ctrl and Tal1−/− mice. * P < 0.05, ** P < 0.01 (paired t-test). Data are representative of at least 5 independent experiments.