FIGURE 3.

Rag2-GFP⁺ LMPPs have B and T lymphocyte but little myeloid potential in vivo. (A) Sub-lethally irradiated CD45.1 host mice were reconstituted with Rag2-GFP⁺ or Rag2-GFP⁻ LMPPs from CD45.2⁺ Ctrl (black) and Tal1−/− (blue) mice. CD45.2⁺ splenocytes and thymocytes from recipient mice were analyzed by FACS 14 days following reconstitution for myeloid cells (Gr-1) and B lymphocytes (CD19) or T lymphocytes (CD4 and CD8). (B) Summary of the percent of CD45.2+ splenocytes that were B lymphocytes or myeloid cells or the percent of thymocytes that were CD45.2⁺. Mice were reconstituted with Ctrl (white) or Tal1−/− (black) LMPPs. N=4. n=5, ** P < 0.01, and **** P < 0.001 (Student’s t-test).