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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: J Immunol. 2019 Apr 8;202(10):2837–2842. doi: 10.4049/jimmunol.1801220

FIGURE 4.

FIGURE 4.

Tal1-deficiency increased the frequency of HSC and LMPPs priming lymphoid genes. (A) Hierarchical clustering of differentially expressed genes in triplicate samples of LSKs sorted on the basis of CD135 and Rag2-GFP expression. (B) Gene set enrichment analysis (GSEA) was used to determine the relative enrichment of CLP-associated (lymphoid) or GMP-associated (myeloid) genes in Rag2-GFP+ LMPPs versus Rag2-GFP− LMPPs. (C) Bar graphs depict normalized relative signal abundance of selected genes from RNA-seq analysis of HSC and LMPP from Ctrl RGFP (+) and Tal1−/− RGFP (–) mice sorted as Rag2-GFP+ or Rag2-GFP−. Data is from 3 independent experiments. * P < 0.05 ** P < 0.01, ***P < 0.001, **** P < 0.0005 in Student’s t-test comparing Rag2-GFP+ and Rag2-GFP− population of the same genotype.