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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: J Immunol. 2019 Apr 8;202(10):2907–2923. doi: 10.4049/jimmunol.1801565

Figure 3. Nur77 limits survival of “naïve” B cells chronically exposed to their cognate antigen in vivo.

Figure 3.

A. Schematic of experimental set up. Splenocytes from IgHEL Tg and WT CD45.1 mice were loaded with the vital dye Cell Trace Violet (CTV) and combined 1:1. 5×106 total splenocytes were adoptively co-transferred into either WT or sHEL Tg hosts via I.V. injection. 3 days after adoptive transfer, mice were sacrificed, and spleens were harvested for surface marker analysis via flow cytometry. Donor cells were identified as CTV+, and IgHEL Tg and WT CD45.1 B cells were separated based on CD45.1 and CD45.2 staining. B. Representative plots shown. C. Ratio of transferred IgHEL Tg cells compared to competitively transferred CD45.1 WT cells normalized to the input ratio. N=3 per group. D. IgMa MFI of IgHEL Tg B cells recovered from adoptively transferred hosts. N=3 per group.