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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: J Immunol. 2019 Apr 5;202(10):2982–2990. doi: 10.4049/jimmunol.1701443

Figure 1. Increased expression of C5aR1 in RAW247.4 macrophages upon knockdown of BVR-A. A-B.

Figure 1.

C5aR1 cell surface expression on BMDM harvested from C57BL/6 mice was measured at day 0–5 of their differentiation with M-CSF by flow cytometry. Data are representative of 3 independent experiments. ***p<0.001 (ANOVA, post-hoc Tukey test) at 1–5 days compared to untreated control cells. C-F. RAW264.7 cells were stably transfected with shRNA against BVR-A (shRNA BVR-A) or shRNA control (Control). Gene (C) and protein expression (D) of BVR were analyzed using RT-PCR and western blot, respectively. Results are representative of three independent experiments (n = 3/group); **p<0.01, *p<0.05 (Mann-Whitney test); control vs shRNA BVR-A. E-F. Gene expression (E) and cell surface expression (F) of C5aR1 were measured by RT-PCR and flow cytometry, respectively, in RAW264.7 shRNA-control and shRNA-BVR-A cells. The data are representative of three independent experiments (n = 3/group). ***p<0.001 (Student T test; p=0.1 using Mann-Whitney test) control vs shRNA BVR-A.