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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2019 Mar 18;30(5):886–892. doi: 10.1007/s13361-019-02187-6

Figure 1.

Figure 1.

Trimethylamine oxide (TMAO) charge reduces and preserves compact, folded states of the ammonium channel (AmtB). a) Chemical structures of tetraethylene glycol monooctyl ether (C8E4) and ndodecyl-N,N-dimethylamine-N-oxide (LDAO) and their respective head groups: tetraethylene glycol (E4) and TMAO. b) Native mass spectra of AmtB in 200mM ammonium acetate (AA) with 2x critical micelle concentration (CMC) C8E4 and increasing amounts of TMAO. The final concentration of TMAO is listed for each mass spectrum. c) Plotted is the average charge state as a function of TMAO concentration. TMAO exhibits a concentration dependent charge reduction. d) IM resolving power increases with lower charge states accessed with TMAO.