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. 2019 May 2;12:3339–3347. doi: 10.2147/OTT.S199369

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© 2019 Lu et al.

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Effects of FASN on CRC cell proliferation and migration in vitro. (A) Colony formation assays were performed in SW480 cells stably transfected with control-shRNA (NC) or shRNA against FASN (KD). Representative photographs are presented (left; magnification, x1) and the colonies were counted (right), presented as the mean±SEM (n=3). (B) Colony formation assays were performed in HCT116 cells stably transfected with empty vector (VEC) or plasmid encoding human FASN (FASN). Representative photographs are presented (left; magnification, x1) and the colonies were counted (right), presented as the mean±SEM (n=3). (C) Migration assays were conducted in SW480 NC and KD cells. Representative photographs are presented (left; magnification, x200) and the number of migratory cells (right) were counted, presented as the mean±SEM (n=3). (D) Migration assays were conducted in HCT116 VEC and FASN cells. Representative photographs are presented (left; magnification, x200) and the number of migratory cells (right) were counted, presented as the mean±SEM (n=3). Statistical significance was determined by a two-tailed, unpaired Student t-test. **P<0.01. *P<0.05.

Abbreviations: FASN, fatty acid synthase; CRC, colorectal cancer; NC, control-shRNA; KD, shRNA against FASN; VEC, empty vector; FASN, plasmid encoding human FASN; SEM, standard error of the mean.