Figure 5.
L. acidophilus and L. rhamnosus reduce S. Javiana virulence gene expression. HT29-MTX cells were pre-treated with or without LAB species L. acidophilus, L. rhamnosus or L. casei (MOE 10) for 1 h prior to infection with S. Javiana (MOI 10). At 2 h post-infection, bacteria were harvested, RNA was isolated and expression of S. Javiana invA, prgH, pltA and cdtB was analyzed via qRT-PCR. The effect of LAB exposure on S. Javiana gene expression was determined using the ΔΔCT method. Data are presented as fold change in gene expression in S. Javiana cells exposed to LAB compared to S. Javiana alone. Genes exhibiting > 3-fold change (denoted by dotted line in figure) were considered altered for expression.