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. 2019 May 7;39(5):BSR20181374. doi: 10.1042/BSR20181374

Figure 2. The expression of RRM2-related miRNAs and miR-20a-5p directly targets RRM2 3′UTR.

Figure 2

(A) Putative binding sites of miRNAs in the RRM2 3′UTR identified using the databases are shown. (B) qRT-PCR analysis of the relative expression of miRNAs in parental or gemcitabine-resistant cells. U6 was used as internal control, and miRNAs expression in daughter cells was normalized to parental cells. (C and D) The indicated wt- or mut-MMR2 luciferase vectors and miR-20a-5p mimic Control/ mimic (C) or miR-20a-5p inhibitor Control/inhibitor (D) was co-transfected into HEK293 cells. The luciferase activity of luciferase reporter vector was then determined. All assays were performed in triplicate and repeated at least three times (mean ± SD); **P<0.01.