Table 2.

Comparison of proteins curated by Ingenuity® Pathway Analysis (IPA) vs manual biocuration. Bolded proteins were common between the two methodologies

Neutrophil category	Proteins curated in IPA	Proteins manually curated
Migration	ALB – albumin ANXA1 – annexin A1 CCR7 – C-C chemokine receptor type 7 CSF2 – colony stimulating factor 2 FN1 – fibronectin 1 HCK – tyrosine-protein kinase HGF – hepatocyte growth factor IL17RA – interleukin 17A receptor INPP5D – inositol polyphosphate-5-phosphatase D ITGAM – integrin alpha M ITGB3 – integrin beta 3 NOS2 – nitric oxide synthase 2 S100A8 – S100 calcium binding protein A8 SERPINC1 – serpin family C, member 1 SLPI – secretory leukocyte peptidase inhibitor TNF – tumor necrosis factor TXN – thioredoxin	alpha-1-acid glycoprotein 2 alpha-1-acid glycoprotein-like alpha-1-antiproteinase 2-like isoform X1 annexin A1 apolipoprotein A-I-like apolipoprotein A-IV C-C chemokine receptor type 7 complement factor B dnaJ homolog subfamily C member 27 EGF-like module-containing mucin-like hormone receptor-like 2-like fibronectin isoform X1 gelsolin granulocyte-macrophage colony-stimulating factor precursor hemopexin integrin alpha-4 integrin alpha-M isoform X1 interleukin-17 receptor A isoform X1 nitric oxide synthase, inducible phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X1 polypeptide N-acetylgalactosaminyltransferase 1 protein S100-A8 prothrombin pulmonary surfactant-associated protein D reactive oxygen species modulator 1-like thioredoxin toll-like receptor 8 precursor tumor necrosis factor tyrosine-protein kinase HCK
Activation	AHR – aryl hydrocarbon receptor ANXA1 – annexin A1 C3 – complement C3 CSF2 – colony stimulating factor 2 EPX – eosinophil peroxidase F2 – coagulation factor II, thrombin FN1 – fibronectin 1 IL18RAP – interleukin-18 receptor accessory protein ITGAM – integrin alpha M LCN2 – lipocalin 2 LTF – lactotransferrin SLPI – secretory leukocyte peptidase inhibitor TNF – tumor necrosis factor	alpha-1-acid glycoprotein 2 alpha-1-acid glycoprotein-like alpha-1-antiproteinase 2-like isoform X1 anionic trypsin annexin A1 annexin A5 isoform X2 apolipoprotein A-I-like apolipoprotein A-IV beta-2-glycoprotein 1 C-C chemokine receptor type 7 complement C2 complement C3 complement component C1q receptor complement factor B cytochrome c dnaJ homolog subfamily C member 27 dystrophin granulocyte-macrophage colony-stimulating factor precursor haptoglobin hexokinase-2 interleukin-18 receptor accessory protein isoform X1 lactotransferrin precursor leptin receptor-like mitogen-activated protein kinase kinase kinase 2 myoglobin nck-associated protein 1-like phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X1 plasma protease C1 inhibitor polymeric immunoglobulin receptor prothrombin ral guanine nucleotide dissociation stimulator reactive oxygen species modulator 1-like serotransferrin precursor thioredoxin toll-like receptor 1 precursor toll-like receptor 8 precursor tropomyosin alpha-1 chain-like tumor necrosis factor
Chemotaxis	APOA1 – apolipoprotein A1 C3 – complement C3 CCR7 – C-C chemokine receptor type 7 CSF2 – colony stimulating factor 2 HCK – tyrosine-protein kinase INPP5D – inositol polyphosphate-5-phosphatase D ITGA4 – integrin alpha 4 ITGAM – integrin alpha M LCN2 – lipocalin 2 NCKAP1L – Nck-associated protein 1 like NOD2 – nucleotide-binding oligomerization domain-containing protein 2 PIGR – polymeric immunoglobulin receptor S100A8 - S100 calcium binding protein A8 SERPINA1 – serpin family A, member 1 SLPI – secretory leukocyte peptidase inhibitor TGFB2 – transforming growth factor-beta 2 THBS4 – thrombospondin-4 TNF – tumor necrosis factor	alpha-1-acid glycoprotein 2 alpha-1-acid glycoprotein-like alpha-1-antiproteinase 2-like isoform X1 amyloid beta A4 precursor protein-binding family A member 2 ankyrin repeat domain-containing protein 11 annexin A1 annexin A2 annexin A5 isoform X2 complement C2 complement C3 complement component C1q receptor fibronectin isoform X1 gamma-aminobutyric acid type B receptor subunit 2 gelsolin granulocyte-macrophage colony-stimulating factor precursor hemopexin hepatocyte growth factor isoformX1 integrin alpha-3 isoform 1 integrin alpha-4 leptin receptor-like microtubule-associated protein 2 isoform X8 nitric oxide synthase, inducible phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X1 plasma protease C1 inhibitor polymeric immunoglobulin receptor protein S100-A8 prothrombin pulmonary surfactant-associated protein D thioredoxin tumor necrosis factor tyrosine-protein kinase HCK tyrosine-protein kinase JAK3 utrophin-like vitamin D-binding protein
Adhesion	ADGRE2 – adhesion G protein-coupled receptor E2 ANXA1 – annexin A1 APOA1 – apolipoprotein A1 C3 – complement C3 CSF2 – colony stimulating factor 2 F2 – coagulation factor II, thrombin FN1 – fibronectin 1 INPP5D – inositol polyphosphate-5-phosphatase D ITGAM – integrin alpha M KNG1 – kininogen 1 LCP1 – plastin 2 NOS2 – nitric oxide synthase 2 S100A8 – S100 calcium binding protein A8 SERPINA1 – serpin family A, member 1 TNF – tumor necrosis factor TXN – thioredoxin	A disintegrin and metalloproteinase with thrombospondin motifs 13 alkaline phosphatase, tissue-nonspecific isozyme isoform X1 alpha-1-antiproteinase 2-like isoform X1 annexin A1 apolipoprotein A-I-like apolipoprotein A-IV C-C chemokine receptor type 7 CD2-associated protein complement C3 dnaJ homolog subfamily C member 27 EGF-like module-containing mucin-like hormone receptor-like 2-like fibrinogen gamma chain fibronectin isoform X1 gelsolin granulocyte-macrophage colony-stimulating factor precursor hemopexin hepatocyte growth factor isoformX1 integrin alpha-4 integrin alpha-M isoform X1 kininogen-1 isoform X2 nitric oxide synthase, inducible plasma protease C1 inhibitor plastin-2 protein S100-A8 thioredoxin tumor necrosis factor tyrosine-protein kinase JAK2 isoform X1
Cell spreading	N/A	apolipoprotein A-I-like apolipoprotein A-IV serum albumin precursor tumor necrosis factor tyrosine-protein kinase HCK
Transmigration	N/A	annexin A1 annexin A2 antithrombin-III isoform X1 catenin alpha-3 isoform 1 C-C chemokine receptor type 7 hepatocyte growth factor isoformX1 integrin beta-3 precursor plasma protease C1 inhibitor polymeric immunoglobulin receptor tumor necrosis factor
Infiltration	ADAMTS13 – ADAM metallopeptidase with thrombospondin type 1 motif 13 ANXA1 – annexin A1 APOA1 – apolipoprotein A1 C2 – complement C2 C3 – complement C3 C4A/C4B – complement C4A/CFB CD36 – platelet glycoprotein 4 CFB – complement factor B F2 – coagulation factor II, thrombin HGF – hepatocyte growth factor IL17RA – interleukin 17A receptor ITGAM – integrin alpha M LCN2 – lipocalin 2 LRRK2 – leucine-rich repeat kinase 2 MAVS – mitochondrial antiviral-signaling protein NOS2 – nitric oxide synthase 2 PDK4 – pyruvate dehydrogenase kinase 4 PLG – plasminogen SERPINA1 – serpin family A, member 1 SERPINC1 – serpin family C, member 1 TNF – tumor necrosis factor	alpha-1-acid glycoprotein 2 alpha-1-antiproteinase 2-like isoform X1 annexin A1 annexin A2 antithrombin-III isoform X1 argininosuccinate synthase aryl hydrocarbon receptor BK potassium ion channel cadherin-1 complement C4-A dystrophin Fc receptor alpha-chain gelsolin granulocyte-macrophage colony-stimulating factor precursor group IIF secretory phospholipase A2 haptoglobin hepatocyte growth factor isoformX1 interleukin-17 receptor A isoform X1 leucine-rich repeat kinase 1 nitric oxide synthase, inducible pancreatic alpha-amylase peroxiredoxin-1 plasma protease C1 inhibitor plasminogen isoform X1 pulmonary surfactant-associated protein D thioredoxin tumor necrosis factor vitamin D-binding protein
Phagocytosis	C3 – complement C3 CD36 – platelet glycoprotein 4 CSF2 – colony stimulating factor 2 HGF – hepatocyte growth factor ITGAM – integrin alpha M LCN2 – lipocalin 2 NOD2 – nucleotide-binding oligomerization domain-containing protein 2 SFTPA1 – surfactant protein A1 SFTPD – surfactant protein D TNF – tumor necrosis factor	alpha-1-acid glycoprotein 2 alpha-1-acid glycoprotein-like alpha-fetoprotein precursor ankyrin repeat domain-containing protein 11 annexin A1 ceramide kinase ceruloplasmin isoform X2 complement C2 complement C3 complement component C1q receptor complement factor B complement factor I Fc receptor alpha-chain ferritin light chain fibronectin isoform X1 gelsolin granulocyte-macrophage colony-stimulating factor precursor hemopexin leptin receptor-like nucleotide-binding oligomerization domain-containing protein 2 plasma protease C1 inhibitor pulmonary surfactant-associated protein A precursor pulmonary surfactant-associated protein D toll-like receptor 1 precursor tumor necrosis factor
Respiratory burst	C3 – complement C3 FN1 – fibronectin 1 HCK – tyrosine-protein kinase ITGA4 – integrin alpha 4 ITGAM – integrin alpha M ITGB3 – integrin beta 3 TNF – tumor necrosis factor	alpha-1-acid glycoprotein 2 alpha-1-acid glycoprotein-like apolipoprotein A-II apolipoprotein A-IV C-C chemokine receptor type 7 complement C2 complement C3 complement component C1q receptor complement factor B dnaJ homolog subfamily C member 27 EF-hand calcium-binding domain-containing protein 3 granulocyte-macrophage colony-stimulating factor precursor group IIF secretory phospholipase A2 haptoglobin integrin alpha-M isoform lactotransferrin precursor leptin receptor-like mitogen-activated protein kinase kinase kinase 2 phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X1 protein S100-A8 pulmonary surfactant-associated protein A precursor pulmonary surfactant-associated protein D thioredoxin toll-like receptor 1 precursor tumor necrosis factor tyrosine-protein kinase HCK
Apoptosis	N/A	alpha-1-acid glycoprotein-like alpha-1-antiproteinase 2-like isoform X1 amyloid beta A4 precursor protein-binding family A member 2 annexin A1 annexin A5 isoform X2 antithrombin-III isoform X1 argininosuccinate synthase bcl-2-related protein A1 cytochrome c dnaJ homolog subfamily C member 27 granulocyte-macrophage colony-stimulating factor precursor lactotransferrin precursor leptin receptor-like nitric oxide synthase, inducible phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X1 protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform thioredoxin tumor necrosis factor