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. 2019 May 1;10:564. doi: 10.3389/fpls.2019.00564

FIGURE 1.

FIGURE 1

The response of the SAC51 promoter and 5’ leader regions to thermospermine. (A) Gene structure of SAC51 and the regions used for GUS fusion constructs. Bars are promoter regions and introns of SAC51. Exons are shown in white boxes in which colored and black areas represent uORFs and a main ORF, respectively. sac51-d contains a premature stop codon in uORF4. (B) GUS staining patterns in cotyledons carrying the GUS fusion with only the SAC51 promoter (upper panel) and that with the SAC51 promoter-5′ leader (lower panel). (C) Relative GUS activity of GUS fusion constructs. Ten-day-old seedlings were treated with or without 100 μM thermospermine for 24 h before GUS assays. Results are from single representative transgenic lines for each construct and the GUS activity of the SAC51 promoter-5′ leader-GUS fusion without thermospermine is set as 1. Error bars represent SD (n = 4). Different letters indicate statistically significant differences between means by two-way ANOVA with Tukey–Kramer multiple comparison test (P < 0.05).