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. 2019 May 1;10:564. doi: 10.3389/fpls.2019.00564

FIGURE 4.

FIGURE 4

Comparison of the conserved uORFs of SAC51 family genes. (A) Alignment of amino acid sequences deduced from the conserved uORFs of SAC51 family genes from different plant species. The amino acids encoded by the internal uORF corresponding to uORF6 of AtSAC51 are colored in red and those encoded by another uORF in SACL3 homologs are shaded. Dashes indicate gaps. At, Arabidopsis thaliana; Bo, Brassica oleracea; Os, Oryza sativa; Pt, Populus trichocarpa; Gm, Glycine max. Gene ID or GenBank accession numbers are given in parentheses. (B) Phylogenetic relationship of the conserved uORFs shown in (A). The tree based on the deduced amino acid sequences was constructed using the neighbor-joining method of the MEGA7 software (Kumar et al., 2016). The scale bar indicates the number of amino acid substitutions per site. (C) The response of each 5′ leader region fused with the CaMV 35S promoter to thermospermine. GUS assays were performed as shown in Figure 1C. Error bars represent SD (n = 4). Asterisks indicate a significant increase as compared with control (t-test, ∗P < 0.05, ∗∗P < 0.01).