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. 2019 May 1;10:853. doi: 10.3389/fimmu.2019.00853

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Copyright © 2019 Valoti, Alberti, Iatropoulos, Piras, Mele, Breno, Cremaschi, Bresin, Donadelli, Alizzi, Amoroso, Benigni, Remuzzi and Noris.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Complement activation on endothelial cells. Endothelial surface area covered by C5b-9 staining after incubation of ADP-activated HMEC-1 with serum from aHUS patients with anti-FHs studied at remission (patients 18 and 20). For each sample, values were expressed as the percentage of C5b-9 deposits induced by a pool of sera from 10 healthy controls run in parallel (reference 100%). The red dashed lines indicated the normal range (60–149%) determined by testing single sera from 35 different healthy controls.