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. 2019 May 1;12:104. doi: 10.3389/fnmol.2019.00104

FIGURE 3.

FIGURE 3

Fluorescence recovery after photobleaching measurements on the lower membrane of HEK293 SNAP-MOP cells following ligand stimulation. (A) Fluorescence recovery curves of HEK293 SNAP-MOP cells during FRAP experiments under control conditions and following stimulation with 30 μM morphine or 10 μM DAMGO for 20 min at 37°C (n = 26–31 from 6 independent experiments). MF, mobile fraction and IF, immobile fraction. (B) Immobile fraction and (C) diffusion coefficient of SNAP-MOP as determined by FRAP (DFRAP) analysis following stimulation with 30 μM naloxone, 30 μM morphine or 10 μM DAMGO (n = 26–31 from 6 independent experiments; one-way ANOVA, F(df1,df2): F(3,114) = 10.48, P < 0.0001). Each data point of the scatter plots represents an individual cell measurement (number of cells in parenthesis) and column plots represent mean ± SEM of the indicated number of cells. ∗ denotes significance vs. vehicle treatment in one-way ANOVA with Sidak’s multiple comparisons test (∗∗∗∗p < 0.0001).