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. 2019 May 1;7:92. doi: 10.3389/fbioe.2019.00092

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Copyright © 2019 Davies, Cox, Azoidis, McGuinness, Cooke, Heaney, Davis, Jones and Grover.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of differentially expressed proteins localized to osteoblast sEVs identified using LC-MS/MS. sEVs were isolated from three separate donors with three repeats performed for each donor. (A) Schematic representation of the relationship between sEVs in relation to the maturity and organization of the mineralized matrix. (B) Venn diagram depicting specific and non-specific proteins localized to sEVs isolated during 1, 2- and 3-weeks mineralization. (C) MS peak intensity plots comparing -Log₁₀ MS-peak intensities of proteins associated with sEVs during weeks 1 (i), 2 (ii), and 3 (iii) of differentiation and their associated Pearson's correlation values. N = 3.