Table 1.
Strains and plasmids used in the study.
| Strain/Plasmid | Genotype | References or source |
|---|---|---|
| 168 | trpC2 | Laboratory stock |
| OAM734 | trpC2 tsaD::Tn (Kmr) | This study |
| OAM735 | trpC2 ylxR (Emr lacZ::Tcr) | Ogura and Kanesaki, 2018 |
| YqfOd | trpC2 yqfO (Emr lacZ) | BSORF |
| OAM737 | trpC2 yqfO (Emr lacZ::Tcr) | This study |
| 501-76 | trpC2 pdhB (Kmr) | Gao et al., 2002 |
| OAM709 | trpC2 thrC::sigX-lacZ (-43 to +2621, Emr) | Ogura and Asai, 2016 |
| OAM811 | trpC2 thrC::sigX-lacZ (-43 to +262, Emr) pdhB (Kmr) | This study |
| OAM812 | trpC2 thrC::sigX-lacZ (-43 to +262, Emr) pta (Tcr) | This study |
| OAM762 | trpC2 thrC::sigX-lacZ (-43 to +262, Emr) acsA (Tcr) | This study |
| OAM813 | trpC2 thrC::sigX-lacZ (-43 to +262, Emr) ackA (Spr) | This study |
| OAM767 | trpC2 thrC::sigX-lacZ (-43 to +262, Emr) yqfO (EmrlacZ::Tcr) | This study |
| OAM874 | trpC2 thrC::sigX-lacZ (-43 to +262, Emr) tsaD::Tn (Kmr) | This study |
| OAM764 | trpC2 thrC::sigX-lacZ (-43 to +262, Emr) tsaB (Tcr) | This study |
| OAM738 | trpC2 thrC::sigX-lacZ (-43 to +262, Emr) amyE::Px-yqfO (Cmr) yqfO (EmrlacZ::Tcr) | This study |
| OAM739 | trpC2 thrC::sigX-lacZ (-43 to +262, Emr) amyE::Px-tsaD (Cmr) tsaD::Tn (Kmr) | This study |
| OAM850 | trpC2 amyE::PtrmK-lacZ (-295 to -12, Cmr) | This study |
| OAM851 | trpC2 amyE::PtrmK-lacZ (-295 to -1, Cmr) ylxR (Kmr) | This study |
| OAM747 | trpC2 thrC::PthiL-lacZ (-500 to -12, Spr) | This study |
| OAM748 | trpC2 thrC::PthiL-lacZ (-500 to -1, Spr) ylxR (Emr lacZ::Tcr) | This study |
| OAM749 | trpC2 thrC::PthiL-lacZ (Spr) yqfO (Em’ lacZ::Tcr) | This study |
| FU1019 | trpC2 lys amyE::PpdhA-lacZ (-167/-471, Cmr) | Tojo et al., 2010 |
| OAM759 | trpC2 amyE::PpdhA-lacZ (-161/-47, Cmr) | This study |
| OAM760 | trpC2 amyE::PpdhA-lacZ (-167/-47, Cmr) tsaD::Tn (Kmr) | This study |
| OAM761 | trpC2 pdhA::pMUT-His-pdhA (Emr) | This study |
| OAM762 | trpC2 pdhA::pMUT-His-pdhA (Emr) tsaD::Tn (Kmr) | This study |
| OAM777 | trpC2 pdhB::pMUT-His-pdhB (Emr) | This study |
| OAM778 | trpC2 pdhB::pMUT-His-pdhB (Emr) tsaD::Tn (Kmr) | This study |
| OAM779 | trpC2 pdhD::pMUT-His-pdhD (Emr) | This study |
| OAM780 | trpC2 pdhD::pMUT-His-pdhD (Emr) tsaD::Tn (Kmr) | This study |
| Plasmid | Description | This study |
| pX | Ampr amyE::xylR -Pxyl Cmr | Hori et al., 2002 |
| pX-yqfO | Ampr amyE::xylR-Pxyl-yqfO (yqfO ORF with its SD), Cmr | This study |
| pX-tsaD | Ampr amyE::xylR-Pxyl-tsaD (tsaD ORF with its SD), Cmr | This study |
| pIS248 | Ampr amyE::lacZ Cmr | Tsukahara and Ogura, 2008 |
| pIS-trmK | Ampr amyE::PtrmK-lacZ (-295 to -12) Cmr | This study |
| pDG1729 | Ampr Em’ thrC::lacZ Spr | Guérout-Fleury et al., 1996 |
| pDG1729-thiL-Wt | Ampr Emr thrC::PthiL-lacZ (-500 to -12) Spr | This study |
| pLacZ::Tc | Ampr lacZ::Tcr | Ogura et al., 2003 |
| pTYB11-ylxR | Ampr intein-ylxR | Ogura and Kanesaki, 2018 |
| pMUTIN-His | Ampr Emr His-tag Pspac | Murayama et al., 2015 |
| pMUT-His-pdhA | Ampr Emr, C-terminal region of pdhA, His-tag Pspac | This study |
| pMUT-His-pdhB | Ampr Emr, C-terminal region of pdhB, His-tag Pspac | This study |
| pMUT-His-pdhD | Ampr Emr, C-terminal region of pdhD, His-tag Pspac | This study |
1Numbers indicate the nucleotide positions ralative to the transcription start point for sigX-lacZ and PpdhA-lacZ. 2Numbers indicate the nucleotide positions ralative to the translation start point for PtrmK-lacZ and PthiL-lacZ. The rests of the strains and plasmids for deleted and mutated PtrmK-lacZ and PthiL-lacZ fusions are listed in Supplementary Table S1.