Table 1.
Primary and secondary antisera.
| Primary antisera | Raised in | Dilution factor | Source |
|---|---|---|---|
| vGluT2 | Guinea pig | 1:1000 | Synaptic Systems |
| vAChT | Rabbit | 1:1000 | Synaptic Systems |
| Synaptophysin | Rabbit | 1:100 | Abcam |
| calb | Rabbit | 1:1600 | SWANT |
| calr | Goat | 1:1000 | SWANT |
| nNOS | Sheep | 1:1000 | Gift from P. Emson |
| Hu | Human | 1:5000 | Gift from Dr. V. Lennon |
| Secondary antisera | Raised in | Dilution factor | Source |
| Anti-Guinea Pig 594 | Donkey | 1:400 | Jackson Immuno Research Labs |
| Anti-Guinea Pig FITC | Donkey | 1:100 | Millipore |
| Anti-Guinea Pig 647 | Donkey | 1:200 | Jackson Immuno Research Labs |
| Anti-Rabbit 488 | Donkey | 1:400 | Molecular Probes |
| Anti-Rabbit 594 | Donkey | 1:400 | Molecular Probes |
| Anti-Rabbit 647 | Donkey | 1:400 | Molecular Probes |
| Anti-Sheep 488 | Donkey | 1:400 | Molecular Probes |
| Anti-Sheep 647 | Donkey | 1:400 | Molecular Probes |
| Anti-Hu 594 | Human | 1:750 | Jackson Immuno Research Labs |