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. 2019 May 7;9:7058. doi: 10.1038/s41598-018-33120-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of vertebrate GPCR clades mapped onto neighbor-joining trees of the α-branch (1/2). AMIN (a,b). Some branches are collapsed together. Species symbol, see Table 1. Squares, genes mistakenly removed during the curation process; circles, sequences of a representative species added in Ensembl.R90-R91 releases. Internal duplications (stars), presence of 3R duplicates (number “2”). Isolated non-assigned sequences (“?”) or genes in Ensembl.R67 that are not found in further releases (“Δ”). Bootstrap values above 65% (black circles). Highlights: new receptors that have (green) or have not (light red) been to our knowledge previously reported; presence of putative ancestral sequences from lamprey (light blue) or early vertebrates or invertebrates (light grey). Some branches have been collated together.