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. 2019 May 7;9:7004. doi: 10.1038/s41598-019-43441-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the transcriptional activity of the 121 promoter with the strong lepidopteran promoters, BmA3 and Bmhsp90. The Bombyx mori promoters and the 121 promoter (−1,333) were ligated to a luciferase reporter gene, and the plasmid vectors were transfected into various insect cells: S2 (a), SaPe-4 (b), AeAl-2 (c), Sf9 (d), BmN4 (e) and Tc81 (f). Forty-eight hours after transfection, luciferase activity was measured and normalized to SEAP activity. Normalized values are expressed as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; n = 3–4 in each group.