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. 2019 May 7;9:7004. doi: 10.1038/s41598-019-43441-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Use of the 121 promoter for the establishment of stably transfected S2 cells. The experimental scheme is shown in (a). Nine plasmid vectors for the expression of AcGFP1/Nluc and ZeoR were transfected into S2 cells. After zeocin selection, the cells were assessed for AcGFP1 fluorescence or luciferase activity. In the AcGFP1-expressing cells, the proportions and MFIs of AcGFP1⁺ cells in the live cell population were analyzed by a CytoFLEX S flow cytometer (b,c). In the Nluc-expressing cells, the luciferase activities were measured (d). The values are expressed as mean ± SD; n = 3–4 in each group. Statistical analysis is presented in Tables 1–4.