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. 2019 Mar 20;218(5):1452–1466. doi: 10.1083/jcb.201812109

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© 2019 Jost and Waters

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Figure 5. — Measurement validation example: using a fluorescent biosensor to measure subcellular pH. To validate measurements, known samples (green) are required. These knowns can be used to characterize the dynamic range, linearity, and repeatability of measurements (magenta) and sources of error in the measurements (blue). For more information about pH measurements, see Grillo-Hill et al. (2014) and O’Connor and Silver (2013).