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. 2019 Feb 26;218(5):1653–1669. doi: 10.1083/jcb.201810138

Figure 1.

Figure 1.

Separate GFP fusion sites in Bnl result in different distribution patterns. (A) Drawing depicting the organization of the ASP and bnl-expressing wing disc cells from third instar larva. DB, dorsal branch; TC, transverse connective. (B) Drawing of a sagittal view showing the tubular ASP epithelium, upper-lower Z-axis, ASP cytonemes that contact the disc bnl-source (green nuclei), and the spatial domains of pntP1 and cut induced by high to low Bnl levels (green; Du et al., 2018a). (C) Schematic map of the Bnl protein backbone showing its conserved FGF domain, signal peptide (SP), and four different GFP insertion sites. (D–H′) Representative images of maximum-intensity projection of lower (wing disc source) and upper (ASP) Z-sections of third instar larval wing-discs expressing CD8-GFP, Bnl:GFP1, Bnl:GFP2, Bnl:GFP3, or Bnl:GFP4 under bnl-Gal4 as indicated. Red, αDlg staining marking cell outlines. (I–K) Representative ASP images showing MAPK signaling (αdpERK, red) zones when Bnl:GFP3endo was expressed under native cis-regulatory elements (I), and when bnl-Gal4 overexpressed Bnl:GFP3 (J) or Bnl:GFP1 (K). In D–K, white dashed line, ASP; white arrow, disc bnl-source; dashed arrow, Bnl:GFP puncta in the ASP; arrowhead, ASP without Bnl:GFP1 puncta. Scale bars: 30 µm.