Figure 7.

Condensin I and II play distinct roles in sister chromatid resolution and compaction. (A) Diagram showing composition of condensin I and II. They contain common subunits SMC2 and SMC4 and unique subunits NCAPG/H/D2 (condensin I) or NCAPG2/H2/D3 (condensin II). (B) Western blotting of NCAPD2 (condensin I; left) or NCAPD3 (condensin II; right) proteins following treatment with the indicated siRNA for 48 h. Actin is shown as a loading control. (C) The proportion of each configuration of the fluorescence reporter in cells depleted of NCAPD2 or NCAPD3. TT75 cells were treated and analyzed as in Fig. S2 I. Data from individual cells are shown in Fig. S5 A. The number of analyzed cells at each point is between 10 and 44 (mean: 26) for NCAPD2 siRNA; 10 and 36 (mean: 23) for NCAPD3 siRNA. (D) The proportion of the pink “resolved” (left) or red “compacted” (right) state is compared over time between control, NCAPD2, and NCAPD3 siRNA. These data were taken from Figs. 3 E and 7 C.