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. 2019 Mar 26;218(5):1706–1724. doi: 10.1083/jcb.201807165

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© 2019 Goel et al.

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Figure 2. — Increased AZ number but reduced AZ size revealed at endo mutant NMJs. (A) Representative NMJs in the indicated genotypes immunostained with antibodies against the AZ protein BRP, with insets below showing individual boutons from the same NMJ. (B) Quantification of neuronal membrane surface area (labeled by HRP) shows a significant increase in endo and endo^RNAi, which is rescued in endo rescue with no change found in vGlut-OE. (C) No change in BRP puncta density is observed in any genotype. (D) Total BRP puncta number per NMJ is significantly increased in endo and endo^RNAi, which scales with the increased neuronal membrane surface area. (E) A significant reduction in the area of individual BRP puncta is observed in endo and endo^RNAi, while no significant change is found in vGlut-OE. Error bars indicate ± SEM (n ≥ 17; one-way ANOVA; Table S1). ****, P < 0.0001; ns, not significant.