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. 2019 Mar 15;218(5):1564–1581. doi: 10.1083/jcb.201704019

Figure 4.

Figure 4.

Translation is required for correct localization of translation factor mRNAs to granules. (A) Schematic of the NIP1-MS2 construct with (sl-NIP1-MS2) or without (NIP1-MS2) a 5′ UTR stem loop to limit translation. Z-stacked images (below) of strains carrying these NIP1 constructs. Bar, 5 µm. (B) Pab1p schematic detailing two point mutations, which impact upon translation initiation. Z-stacked images (below) for NIP1-MS2 mRNA in pab1Δ strains bearing wild-type PAB1, PAB1 lacking the RRM2 region, or PAB1 with Y83V and F170V mutations. Bar, 4 µm. (C) Z-stacked images of NIP1-MS2 mRNA in untreated or 1,6-hexanediol treated cells. Bar, 4 µm. (D) Bar chart depicting the impact of stem loop insertion, PAB1 mutation, or hexanediol on NIP1 mRNA granule integrity (n = 3, >100 cells per repeat). Error bars = +SD.