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Granules house translationally active mRNAs. (A) Schematic for the TRICK strategy. Z-stacked images showing NIP1-TRICK and TIF4631-TRICK in rich media (B), after 10 min glucose depletion (C), or in 100 µg/ml cycloheximide (CHX; D). (E) Chart evaluating granules showing both GFP and mCherry signal under each condition (n = 3, >100 cells per repeat). ****, P < 0.0005. Error bars = +SD. Bars, 4 µm.
