Figure 7.

MACF1 is a synaptic podosomal protein. (A) MACF1 (green) concentrates within perforations of AChR clusters (red). Bar, 10 µm. (B) Higher magnification images show that MACF1 (green) is enriched in the core of synaptic podosomes, like F-actin (cyan) and not found at the cortex of the podosome, marked by Vinculin (cyan). Bar, 1 µm. (C) Together with MACF1, MAP1b and EB1 (each shown in green) are enriched in the core of the synaptic podosome. Rapsyn (green) is not found in the perforations but together with AChRs. Bar, 4 µm.