Fig. 5.

W. bancrofti sheath antigen recognizes TLR-4 on dendritic cells. a Co-immunoprecipitation of microfilarial sheath antigen (FSAg)–TLR4 complex followed by reciprocal immunoblotting using anti-FSAg or anti-TLR4 antibodies. b Binding of FITC-labelled FSAg with dendritic cell-TLR4. Dendritic cells were incubated with FITC-conjugated FSAg. In a separate set of experiments, dendritic cells were pre-treated with anti-TLR4 antibodies followed by incubation with FITC-conjugated FSAg (TLR4 Ab + FSAg). Cells were analyzed by confocal microscopy (scale bar = 25 μm). Nuclei were stained with Hoechst staining. Abbreviations: Ctr, control; IP, Immunoprecipitation; IB, Immunoblot