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. 2019 May 7;9:7006. doi: 10.1038/s41598-019-43411-3

Figure 2.

Figure 2

Uptake and localization of the HoThyRu/DOTAP nanosystem in MCF-7 cells. Confocal microscopy bioimaging of cellular uptake of the fluorescent HoThyDansRu/DOTAP nanosystem in MCF-7 cells incubated for 0.5, 1, 2, 4 and 6 h at 100 µM. Nuclei blue fluorescence is due to DAPI (Ex358, Em461 nm). Green dots within cells represent dansyl-associated fluorescent emission (DANS) related to HoThyDansRu/DOTAP (Ex370, Em450–550 nm). E-cadherine-associated red fluorescence (CAD) defines cell shape (Ex650, Em668 nm). In merged images (Merge), the fluorescent patterns from cell monolayers are overlapped. The shown images are representative of three independent experiments. 100× total magnification (10× objective and a 10× eyepiece).