Fig. 1.

GSDMD-deficient macrophages undergo apoptosis after inflammasome activation. a–d TEPMs (a) and BMMs (b–d) of the indicated genotypes were transfected with 150 ng of Poly dA:dT (a) or infected with S. Typhimurium at an MOI of 5 (b–d). Bacteria were washed out (c) or not washed out (b, d), and gentamicin was added to the BMM cultures at 1 h after infection. Cell death was monitored by LDH release assay (a, b). Graphs depict the mean ± SD of triplicate cultures. ***p < 0.001 (Bonferroni’s multiple comparisons test). Microscopic images (c). Western blot detection of activated caspases in culture supernatants plus cell lysates (Sup + CL) (d). e–g RAW264.7 cells of the indicated genotypes were infected with S. Typhimurium at an MOI of 40. Bacteria were washed out, and gentamicin was added to the cultures at 2 h after infection (e). S. Typhimurium-infected Gsdmd-KO RAW264.7 cells showed apoptotic morphological changes. Western blot detection of caspase-3 p17 in cell lysates (f) and Sup + CL (g). Data are from one representative of three biologically independent experiments with similar results (a–g). Source data are provided as a Source Data file. (See also Supplementary Figs. 1 and 2.)