Fig. 10.

Caspase-1 induces apoptosis in primary cortical neurons and mast cells. a Quantitative RT-PCR analysis of GSDMD mRNA expression in BMMs, cortical neurons, and bone marrow-derived mast cells. GSDMD mRNA expression was calculated relative to GAPDH mRNA (the ΔCt method). Data are from three biologically independent experiments. b Primary cortical neurons were exposed to OGD for 8 and 16 h, and the cells were processed for immunofluorescent labeling of cleaved caspase-3 (red), β III-tubulin (green), and nuclei (blue). Representative fluorescent microscopic images from three biologically independent experiments are shown. The graph depicts percentages of cleaved caspase-3⁺ cells (six fields, >900 cells per group). c Surface expression of FcεRIα and c-kit in mast cells was determined by flow cytometry. d–g Mast cells of the indicated genotypes were primed with LPS (100 ng ml⁻¹) for 3 h and then stimulated with nigericin (10 μM) for 0.5 h (e–g) or 1 h (d). Flow cytometric analysis of PI uptake and PS exposure (d). Western blot detection of caspases, Bid, and GSDMs in cell lysates (e–g). In c–g, representative flow cytometry profiles (c, d) and Western blot images (e–g) from three biologically independent experiments are shown. In d, percentages of Annexin V⁺ cells and Annexin V⁺ PI⁺ cells (n = 9) are plotted. In a, b, and d, columns and bars represent the mean ± SD. Statistical significance was determined using the Bonferroni’s multiple comparisons test (a, b, and d). ***p < 0.001. Source data are provided as a Source Data file