Fig. 4.

Caspase-2, 6, 7, and 8 are dispensable for caspase-1-induced apoptosis. a Gsdmd-KO and Gsdmd/Casp2-DKO CL26-iCasp1 cells were stimulated with 50 nM AP20187 for 1.5 h, and cleaved caspase-2 was detected by Western blotting. b Western blot detection of cleaved caspases in WT and Gsdmd-KO CL26-iCasp1 cells treated with AP20187 for the indicated times. c, d, e Gsdmd-KO and Gsdmd/Casp3-DKO CL26-iCasp1 cells (c, d) and RAW264.7 cells (e) were treated with AP20187 and infected with S. Typhimurium as in Fig. 1f, respectively. Cleaved caspases were detected by western blotting. f–i CL26-iCasp1 cells of the indicated genotypes were treated with AP20187 for the indicated times (f, h) or 4 h (g, i). Western blot detection of cleaved caspase-3 and procaspases in the cells (f, h). LDH release from the cells (g, i). Graphs depict the mean ± SD of triplicate cultures, and individual data values are plotted. Data are from one representative of three independent experiments with similar results (a–i). Source data are provided as a Source Data file. (See also Supplementary Fig. 6)