Fig. 9.

Caspase-1 induces apoptosis in L929 cells. a, b L929 cells, L929-vector control cells, and L929-iCasp1 cells were treated with 50 nM AP20187 for the indicated times. Caspases, Bid, and GSDME were detected by Western blotting (a). Flow cytometric analysis of PI uptake and PS exposure (b). Representative flow cytometry profiles and the graph that shows percentages of Annexin V⁺ cells (n = 5) from two independent experiments are shown. Horizontal and vertical bars indicate the mean ± SD. c–e L929-iCasp1 cells were transduced or not transduced with red fluorescent protein 657 (RFP) or GSDMD-RFP, and then treated with AP20187 for the indicated times. Microscopic images (c). Caspases, GSDMD, GSDME, and Bid were detected by Western blotting (d). LDH release (e). The graph depicts the mean ± SD of triplicate cultures. In b and e, statistical significance was determined using the Bonferroni’s multiple comparisons test. ***p < 0.001. In a and c–e, data are from one representative three independent experiments with similar results. Source data are provided as a Source Data file. (See also Supplementary Fig. 13.)