Table 3.
List of genes and isoenzymes used
| Markers | Nature | Function | Primer sequences (5'-3') | RE | LG | References |
| ENBP1 | SSR | Chloroplastic protein | L: CACTTCCCACTGTCCTAGTCCTAC R: GACTCGTCATCACCAGTTTCATCC | 1 | [65]* | |
| ENOD8 | CAPS | Nodulin | L: ATGGTGCAAACTTCGCATCAGGAGG R: ACAACCCTTTGGTCCTGTGCCGTG | SspI | 1 | [66] |
| MtPT-1 | CAPS | Phosphate transporter | L: TTGCTAAGAACCCGAAACAAGCTGC R: ATGTTCATGATCCTTCTTGCTCAAGC | RsaI | 1 | [13] |
| PEPC | CAPS | PhosphoEnolPyruvate Carboxylase | L: CTCATCCTACTCAGTCGGTTCGTCG R: ACACGTAGCTCATCGTTGCAACGCC | RsaI | 2 | [67] |
| Z-F1 | RFLP | Zn-Finger1 | EcoRI | 3 | [68] | |
| GS.B | PCR | Glutamine synthetase | L: AGAAGTAGCGTGGTGGC R: GCTGCCTATGGAGAAGG | 3 | [69] | |
| Mtlec2 | SSR | Lectine 2 | L: AAAGAATTCCATCTTCCAAGGCGA R: GAAGTCTAGAAACCAATCCTTACCC | 3 | [70] | |
| ENOD12 | PCR | Nodulin | L: CCTGCTTATAGGCCACCAC R: CTTCTGCTGGAGGATGCC | 3 | [71] | |
| ATPase | SSR | Vacuolar ATPase | L: GGGTTTTTGATCCAGATCTT R: AAGGTGGTCATACGAGCTCC | 4 | [15]** | |
| MtST-1 | PCR | Sucrose transporter | L: ATCAAAGGTGGATGGGGATGGAG R: TAGACCAGAAGGGATGTGATTTCC | 4 | [72] | |
| PGM | Isoenz. | PhosphoGlucoMutase | 4 | [34] | ||
| Gs.C | CAPS | Glutamine synthetase | L: GCACAAGGAGCACATTG R: GCCCATAATTAAACATCATG | VspI | 5 | [69] |
| Lb I | CAPS | Leghemoglobin I | L: GATAGTCCTCAACTCCAAGC R: CCTGTTGCTCGAAGTTGAGA | SspI | 5 | [73] |
| rDNA | CAPS | 45s Ribosomal DNA | L: ATGGTCCGGTGAAGTGTTCG R: CCCGGTTCGCTCGCCGTTAC | XbaI | 5 | This paper |
| ENOD40 | CAPS | Nodulin | L: TTAGTAGGATCTTCTCTTTCACTAGC R: GCCTCCGATTATCAAAGGTCAAG | AluI | 5 | [74] |
| GS.A | PCR | Glutamine synthetase | L: AAAGACAAGTGGTTGTGAC R: GCTGCCTATGGAGAAGG | 6 | [69] | |
| MtN1 | PCR | Nodulin | L: ATCTATCTTCTTCGCTC R: TGACTGTTGAACATCTC | 6 | [75] | |
| GSHS | SSR | Glutathione synthetase | L: AGCAATAGGCAATGGCTGCTCCTGC R: AATGGTGAGATGCTTATGATGAGAC | 7 | [76] | |
| VR | CAPS | Vestitone reductase | L: GAGTGTGTGTAACTGGAGGTACAGG R: ATGCCTAATGCGCCATCGACGGTTC | DdeI | 7 | [77] |
| PGD | Isoenz. | PhosphoGlucoDeshydrogenase | 7 | [34] | ||
| ENOD16 | RFLP | Nodulin | L: ATCGGGACACTCTTTAGCAAGACCC R: TGGGCGGTGGAATTGGACCTCTTG | EcoRV | 8 | [78] |
Underlined markers have been used for synteny studies RE : Restriction Enzyme LG : M. truncatula Linkage Group SSR : Simple Sequence Repeat: polymorphism is revealed by the length of the amplified fragment CAPS : Cleaved Amplified Polymorphic Sequence : polymorphism is revealed after digestion of the amplified fragment with restriction enzymes PCR : Polymerase Chain Reaction : polymorphism is revealed by the length of the amplified fragment L : Left primer R : Right primer *: Accession n° AJ002479 ** : Accession n° AA660456